would you like to do...和would/will you please do...的区别是什么?

前者是“你想要做...”是单纯的问题,比如“你想要喝牛奶吗?”：
Would you like to have some milk?
后者是“请你做...好吗?”是比较客气的请求（祈使句的委婉形式）,比如“请你坐下好吗?”（有人挡住你的视线）：
Will you please sit down?